Journal:

Article Title: Promoters P3 , Pa / Pb , P4 , and P5 Upstream from bla TEM Genes and Their Relationship to ?-Lactam Resistance

doi: 10.1128/AAC.46.12.4035-4037.2002

Figure Lengend Snippet: β-Lactam susceptibility and β-lactamase activity of TEM-1- and TEM-30 (IRT-2)-producing E. coli transformants according to the type of promoter upstream from the bla TEM-1B and bla TEM-30B genes

Article Snippet: This difference suggests that other nucleotide motifs than TTG in the promoter region might be involved in gene expression. table ft1 table-wrap mode="anchored" t5 caption a7 Strain and bla TEM gene (promoter) MIC (μg/ml) a β-Lactamase activity (U/mg) (mean ± SD) AMC (≤4->16) TIC (≤16->64) TCC (≤16->64) PIP (≤8->64) TZB (≤8->64) CEP (≤8->32) E. coli ATCC 25 922 4 4 4 2 1 8 ND b NM554 2 2 2 1 1 2 ND Transformant NM554 bla TEM-1B ( P3 ) 32 8,192 32 128 1 8 6.8 ± 3.3 bla TEM-1B ( Pa/Pb ) 128 >8,192 256 512 2 32 13.8 ± 2.1 bla TEM-1B ( P4 ) 512 >8,192 1,024 1,024 128 64 38 ± 9 bla TEM-1B ( P5 ) 1,024 >8,192 2,048 >1,024 512 128 87.4 ± 8 bla TEM-30B ( P3 ) 256 128 32 8 1 8 14 ± 4.4 bla TEM-30B ( Pa/Pb ) 2,048 512 128 64 2 8 32.6 ± 5.6 bla TEM-30B ( P4 ) 4,096 2,048 512 64 32 8 120 ± 7.9 Open in a separate window a Drug abbreviations: AMC, amoxicillin with 2 μg of clavulanate per ml; TIC, ticarcillin; TCC, ticarcillin with 2 μg of clavulanate per ml; PIP, piperacillin; TZB, piperacillin with 4 μg of tazobactam per ml; CEP, cephalothin.

Techniques: Activity Assay

Journal: ACS Omega

Article Title: Phage Display-Derived Peptide for the Specific Binding of SARS-CoV-2

doi: 10.1021/acsomega.1c04873

Figure Lengend Snippet: Peptide 1 can specifically bind to the SARS-CoV-2 virus. (a) Schematic of the experimental procedure for the characterization of peptide binding with the heat-inactivated SARS-CoV-2 virus using the magnetic bead assay and TEM. (b–e) Biotinylated peptide 1 was immobilized on SA-coated magnetic beads to capture the virus. The gold nanoparticle-labeled SARS-CoV-2 antibody was used to detect the virus. The viruses and gold nanoparticles were visualized using TEM. The influenza virus served as a negative control. (b,c) Four individual magnetic beads with the addition of 0.67 and 0.067 mg/mL of the SARS-CoV-2 target to the surface, showing that gold nanoparticles (dashed red circle) have conjugated onto the magnetic beads. (d,e) Four individual magnetic beads with the addition of 0.67 and 0.067 mg/mL of influenza virus. The scale bar is 100 nm.

Article Snippet: The SARS envelope protein antibody used in the TEM assay was purchased from Novus Biologicals, LLC., Centennial, CO. N-Hydroxysuccinimide (NHS)-activated gold nanoparticles with a diameter of 40 nm were purchased from Cytodiagnostics Inc., Canada.

Techniques: Virus, Binding Assay, Magnetic Beads, Labeling, Negative Control